Estrogen Promotes the Development of Mouse Cumulus Cells in Coordination with Oocyte-Derived GDF9 and BMP15
نویسندگان
چکیده
منابع مشابه
Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells.
Oocyte-derived bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are key regulators of follicular development. Here we show that these factors control cumulus cell metabolism, particularly glycolysis and cholesterol biosynthesis before the preovulatory surge of luteinizing hormone. Transcripts encoding enzymes for cholesterol biosynthesis were downregulated in bot...
متن کاملIncreased GDF9 and BMP15 mRNA levels in cumulus granulosa cells correlate with oocyte maturation, fertilization, and embryo quality in humans
BACKGROUND Oocyte secreted factors (OSFs), including growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), play an important role in the process of follicular development and oocyte maturation. Since OSFs are expressed in oocytes and cumulus granulosa cells, the aim of the present study was to explore whether the expression levels of GDF9 and BMP15 mRNAs in cumulus g...
متن کاملOocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells.
Mammalian oocytes are deficient in their ability to carry out glycolysis. Therefore, the products of glycolysis that are necessary for oocyte development are provided to oocytes by companion cumulus cells. Mouse oocytes secrete paracrine factors that promote glycolysis in cumulus cells. The objective of this study was to identify paracrine factors secreted by oocytes that promote glycolysis and...
متن کاملThe development of mouse early embryos in vitro in fibroblasts and cumulus cells co-cultures supplemented with retinoic acid
This study was designed to examine the effects of retinoic acid adding to cumulus and/or fibroblast cellsmonolayer on the development of mouse early embryos. One-cell mouse embryos were obtained from NMRImice after superovulation by an intraperitoneal injection of 5 IU equine chorionic gonadotrophin (eCG)followed 48 hrs later by 5 IU human chorionic gonadotrophin (hCG). Mouse embryonic fibrobla...
متن کاملP-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles
Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...
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ژورنال
عنوان ژورنال: Molecular Endocrinology
سال: 2010
ISSN: 0888-8809,1944-9917
DOI: 10.1210/me.2010-0260